Can you run 100% water through C18 column?

It is definitely not recommended to run a common C18 column with pure aqueous mobile phase. Because of their length the linked chains have hydrophobic properties and might collapse if used with 100 % water.

Is C18 reverse or normal phase?

A C18 column is an example of a “reverse phase” column. Reverse phase columns are often used with more polar solvents such as water, methanol or acetonitrile.

What is difference between ODS and BDS?

ODS and BDS are two columns used for reverse-phase chromatography. The key difference between ODS and BDS column is that ODS column contains free –OH functional groups, whereas BDS column contains deactivated –OH groups. Moreover, ODS columns have high peak tailing while BDS columns are designed to reduce peak tailing.

How do you clean a C18 column?

In your case wash the column with 70% water; 15% methanol; 15% acetonitrile. Divert the column eluent to waste not to contaminate your detector(s). Wash the column slowly over to 100% methanol and wash for at least 15 minutes. Wash the column over to 100% acetonitrile and wash for at least 15 minutes.

How do you clean a BEH C18 column?

Purge the pumping system of any buffer-containing mobile phases and connect the inlet end of the column to the injector outlet. 2. Flush column with 100% organic mobile phase (methanol or acetonitrile) by setting the pump flow rate to 0.1 mL/min and increase the flow rate to 0.5 mL/min over 5 minutes.

What is BDS and ODS?

Can we use DMSO in HPLC?

No, you cannot use DMSO in RP chromatogrraphy. It is not soluble in Water. You can use it in GPC or Normal Phase chromatography.

What is BEH C18 column?

ACQUITY UPLC® BEH C18 and C8 columns were designed to be the universal columns of choice for most UPLC® separations by providing the widest pH range.They incorporate trifunctional ligand bonding chemistries which produce superior low pH stability and ultra-low column bleed.