How do you calibrate a disposable pipette?

Pre-rinse the tip by aspirating and dispensing the set volume three times and push fully to remove any remaining liquid. Aspirate the calibration volume without bubble formation and dispense the liquid slowly into the weigh boat. Then, record the weight on the balance and repeat the process ten times.

How often should you calibrate pipettes?

every 3-6 months
According to the Clinical & Laboratory Standards Institute (CLSI), you should calibrate your pipettes every 3-6 months or before they’re put into service. If you fail to do so, then pipette readings may become inaccurate and unreliable.

How do I know my pipetting is accurate?

The most common way to check your pipette accuracy is by weighing water. The density of water is 1 g/mL. This means that every microliter (µL) should weigh 0.001 g. In other words, if your pipette is accurate, the amount of water you dispense will equal the amount the water weighs.

Why is it important to calibrate a pipette?

Pipette calibration is necessary to prevent inaccuracies in order to attain better results, performance, and longevity of the pipette itself.

How do I make my pipette reading more accurate?

How good are you?

1. Pre-wet the pipette tip.
2. Immerse the tip to the proper depth during aspiration.
3. Pause consistently after aspiration.
4. Use consistent plunger pressure and speed.
5. Pull the pipette straight out.
6. Examine the tip BEFORE dispensing a sample.
7. Examine the tip AFTER dispensing a sample.
8. Use standard mode pipetting.

When should the pipettes be calibrated?

every 3 to 6 months
The Clinical and Laboratory Standards Institute (CSLI) recommends that pipettes (single and multi-channel) and automated liquid handlers be calibrated every 3 to 6 months. A minimum of two volumes must be tested (nominal and lowest setting) with ten replicates at each volume.

How much does it cost to calibrate a pipette?

Pipette Calibration Systems Cost Analysis

Pipette Calibration System	Initial one time cost
Gravimetric	Analytical balance (Estimate $3000-$4000)
Photometric	Spectrophotometer (Estimate $1500 -$2,000)
Artel- PCS	PCS instrument ($6,500 – 7,000)
Streck Pipette Verification Service	None