How do you extract DNA from a strawberry step by step?

Experimental Procedure:

1. STEP 1: Mix the DNA Extraction Liquid. Take 1 plastic cup and mix together 2 teaspoons of dish detergent.
2. STEP 2: Get the DNA.
3. STEP 3: Separate the Fluids from the Solids.
4. STEP 4: Extract the DNA.
5. STEP 5: Measure the Amount of Extractable DNA.
6. STEP 6: Repeat Process with Other Types of Strawberries.

How do you make an extraction buffer for strawberry DNA lab?

In a container, add 900mL of water, 50mL of dishwashing detergent (or 100mL shampoo), and finally 2 teaspoons of salt. Slowly invert the bottle to mix the extraction buffer. Note: A modification can be made based on the needs of the students.

How do you extract the most DNA from a strawberry?

Pull off any green leaves on the strawberry that have not been removed yet. Put the strawberries into the plastic bag, seal it and gently smash it for about two minutes. Completely crush the strawberries. This starts to break open the cells and release the DNA.

Why is strawberry so useful to Visualise DNA in extractions?

Ripe strawberries are an excellent source for extracting DNA because they are easy to pulverize and contain enzymes called pectinases and cellulases that help to break down cell walls. And most important, strawberries have eight copies of each chromosome (they are octoploid), so there is a lot of DNA to isolate.

How much DNA can you extract from a strawberry?

Each little piece of a living thing, known as a cell, has DNA in it. In humans each of these cells have 2 copies of the DNA, but in strawberries each of these have 8 copies of the DNA (scientists call this octoploid). That means strawberries have 4 times as many copies of DNA as humans, making it 4 times easier to see!

Why is dish soap used in DNA extraction?

Soap contains a compound called sodium laurel sulfate that removes fats and proteins. Soap will also cut through cell walls because the membranes surrounding the cells are made up of fats and proteins. The dish soap pulls apart the membranes, releasing the DNA.

Why is ethanol used in DNA extraction?

The main role of monovalent cations and ethanol is to eliminate the solvation shell that surrounds the DNA, thus allowing the DNA to precipitate in pellet form. Additionally, ethanol helps to promote DNA aggregation. Usually, about 70 percent of ethanol solution is used during the DNA washing steps.

Why do we add alcohol in DNA extraction?

Lab technicians can add ethanol or isopropyl alcohol (rubbing alcohol) so that the DNA clumps and form a visible white precipitate. It’s important to use cold alcohol because it allows a larger amount of DNA to be extracted. If the alcohol is too warm, it may cause the DNA to denature [bold], or break down.

Can you look at strawberry DNA under a microscope?

Because of the special characteristics of strawberries, it’s possible to extract, isolate and observe a strawberry’s DNA in a matter of minutes without an electron microscope.

How do you analyze strawberry DNA?

Pour a small amount (three to four milliliters) of the filtered strawberry solution into a test tube. Tilt the tube and pour an equal amount of cold isopropyl alcohol into the test tube. The DNA will precipitate to the top of the solution and will resemble a white, fluffy cloud.

Why do strawberries have 8 copies of DNA?

We use strawberries instead of other fruits because they have even more DNA! Each little piece of a living thing, known as a cell, has DNA in it. In humans each of these cells have 2 copies of the DNA, but in strawberries each of these have 8 copies of the DNA (scientists call this octoploid).

Why is alcohol cold in DNA extraction?

Using ice-cold water and ice-cold alcohol will increase your yield of DNA. The cold water protects the DNA by slowing down enzymes that can break it apart. The cold alcohol helps the DNA precipitate (solidify and appear) more quickly.