How does SYTO 9 work?

How does SYTO 9 work?

SYTO 9 binds to nucleic acid, meaning that the amount of nucleic acid in a cell influences the amount of SYTO 9 that will bind and subsequently the fluorescence intensity. Bacterial nucleic acid content was considered with respect to observed differences in SYTO 9 fluorescence.

How do nucleic acid stains work?

SYBR gold, that is. It’s a fluorescent nucleic acid stain, meaning that it binds to nucleic acids (RNA & DNA) and gives off light at a specific wavelength when you shine light of a different specific wavelength at it.

Is SYBR green cell permeable?

The membrane-permeable Sybr Green I molecule (Fig. 3A) carries a single positive charge, which is surrounded by hydrophobic aromatic rings. In contrast, the propidium molecule carries two positive charges, one of which appears open to the surroundings and should prevent its membrane permeation (Fig. 3B).

What other stains can be used to visualize nucleic acids and what are their sensitivities?

5 Common Dyes for Visualizing and Staining DNA

  • Ethidium Bromide. Ethidium bromide is likely the most well-known dye used for visualizing DNA.
  • SYBR Gold. SYBR Gold dye can be used to stain double or single-stranded DNA​ or to stain RNA.
  • SYBR Green.
  • SYBR Safe.
  • Eva Green.

Is SYTO 9 toxic?

As shown in Figure 1, SYTO® 9 is toxic to cells over less than half of the experimental time, whereas MycoLight™ Red JJ94 showed very minor variation from the control population.

Does SYTO 9 stain dead cells?

SYTO 9 stain is particularly used for staining live and dead Gram-positive and Gram-negative bacteria.

What stain is used for DNA?

Ethidium bromide (EtBr)
Ethidium bromide (EtBr). As mentioned above, EtBr used to be the mainstay for staining DNA gels. It is an inexpensive dye (cost per gel ~ $0.30), can detect as little as 1 ng of DNA, and can be used during or after electrophoresis with little to no destaining required.

Why is it necessary to use a DNA stain?

Research laboratories commonly use fluorescent DNA stains because they are extremely sensitive, making it easy to quantify small amounts of DNA. In order to visualize the DNA fragments, an ultraviolet (UV) light source (such as a transilluminator) is used to excite the fluorescent molecules.

What is SYTO?

SYTO ® dyes are cell-permeant nucleic acid stains that show. a large fluorescence enhancement upon binding nucleic acids. The SYTO dyes can be used to stain RNA and DNA in both live. and dead eukaryotic cells, as well as in Gram-positive and Gram- neg a tive bacteria.

What does SYTO mean?

Acronym. Definition. SYTO. Student Youth Travel Organization. Copyright 1988-2018, All rights reserved.

What does SYTO 9 stain?

SYTO 9 stain products SYTO 9 stain is particularly used for staining live and dead Gram-positive and Gram-negative bacteria. Live/dead analysis of Streptomyces antibioticus ETH7451 cultures.

Which stain is used for RNA?

GREEN STAIN is a versatile product line for the detection of nucleic acids (DNA and RNA) in electrophoretic gels.

What is the most specific and most reliable stain for DNA?

Fluorescent stains are the most commonly used DNA stains in research labs because they are very sensitive, meaning they can detect small amounts of DNA. Fluorescent stains are easy to use and are typically added to the gel when it is being poured.

Which stain is used for DNA?

Ethidium bromide
Ethidium bromide is excellent for staining DNA in agarose gel electrophoresis. It can also be used to detect dsDNA in PCRs. Upon binding to DNA, EtBr experiences a roughly 20-fold increase in brightness.

Is EtBr positively charged?

EtBr is positively charged and will run the opposite direction from the DNA.