What are IEC 6 cells?

What are IEC 6 cells?

In terms of iron absorption, IEC-6 cells have a villous enterocyte phenotype and are regulated by iron stores as occurs in vivo; therefore, they represent an appropriate cell model with which to study this process.

What are the 6 cells?

6 Cell Organelles

  • Nucleus. Known as the cell’s “command center,” the nucleus is a large organelle that stores the cell’s DNA (deoxyribonucleic acid).
  • Ribosomes. Ribosomes are the protein factories of the cell.
  • Endoplasmic reticulum.
  • Golgi apparatus.
  • Chloroplasts.
  • Mitochondria.

What is Vero E6 cell line?

VERO C1008 [Vero 76, clone E6, Vero E6] is a cell line exhibiting epithelial morphology that was isolated from the kidney of an African green monkey. This line is a clone of VERO 76 (ATCC CRL-1587). It was cloned by the dilution method into microtiter plates in 1979 by P.J. Price.

Where is ATCC?

Manassas, Virginia
Our newly expanded headquarters and bioproduction facility is located in Manassas, Virginia, near Washington DC, and our FDA-registered biorepository facility and research and development center is located in Gaithersburg, Maryland.

Do VERO e6 cells express TMPRSS2?

The messenger RNA expression level of TMPRSS2 in VeroE6/TMPRSS2 cells is ∼10-fold higher than in normal human lung tissue and other human cell lines (Fig. 1A). SARS-CoV-2 uses the same receptor, ACE2, as SARS-CoV (2), and ACE2 expression is very high in VeroE6 cells (7).

Why do we use Vero cells?

In addition, Vero cells have been licensed in the United States for production of both live (rotavirus, smallpox) and inactivated (poliovirus) viral vaccines, and throughout the world Vero cells have been used for the production of a number of other viruses, including Rabies virus, Reovirus and Japanese encephalitis …

What are TMPRSS2 inhibitors?

The host cell serine protease TMPRSS2 is an attractive therapeutic target for COVID-19 drug discovery. This protease activates the Spike protein of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and of other coronaviruses and is essential for viral spread in the lung.

What type of cells are Vero cells?

Vero cells are a lineage of cells used in cell cultures. The ‘Vero’ lineage was isolated from kidney epithelial cells extracted from an African green monkey (Chlorocebus sp.; formerly called Cercopithecus aethiops, this group of monkeys has been split into several different species).

What is Camostat mesylate?

Camostat mesylate—one of many types of COVID-19 treatments under study—targets a human protein named TMPRSS2 that helps the virus enter a human cell. Its normal function in the cell is unknown.

What are HuH-7 5 cells?

The human hepatoma-derived HuH-7 cell line and its derivatives (Huh7. 5 and Huh7. 5.1) have been widely used as a convenient experimental substitute for primary hepatocytes. In particular, these cell lines represent host cells suitable for propagating the hepatitis C virus (HCV) in vitro.