What influences enzyme activity lab?

The main factors which will affect the activity of an enzyme on a given substrate are:

• Temperature (use water baths to minimise fluctuations)
• pH (acidic or alkaline solutions)
• Substrate concentration (choose range to avoid saturation)
• Presence of inhibitor (type of inhibitor will be enzyme-specific)

How do you measure enzyme activity in a lab?

Enzyme units

1. The quantity or concentration of an enzyme can be expressed in molar amounts, as with any other chemical, or in terms of activity in enzyme units.
2. Enzyme activity = moles of substrate converted per unit time = rate × reaction volume.

What was the purpose of the enzyme lab?

Abstract: The purpose of this lab is to investigate the impact of temperature, substrate concentration, enzyme concentration, and the presence of an inhibitor on the effectiveness and rate of an enzyme. If the concentration of the enzyme or substrate is increased, the rate of the reaction will be increased as well.

What is the enzyme activity?

Enzyme activity is measured in units which indicate the rate of reaction catalysed by that enzyme expressed as micromoles of substrate transformed (or product formed) per minute.

What 2 factors affect the rate of enzyme activity?

Enzymes are affected by the hydrogen ion concentration (pH) and the temperature. Enzymes are highly specific compared to other catalysts, and each enzyme is specialized for one reactant substance.

What factors increase enzyme activity?

Factors Affecting Enzyme Activity

• Factor 1: Concentration of Enzyme.
• Factor 2: Concentration of Substrate.
• Factor 3: Effect of Temperature.
• Factor 4: Effect of pH.
• Factor 5: Effect of Activators.

How pH regulate enzyme activity?

PH not only affects the activity of the enzyme, but also affects the charge and shape of the substrate, so that the substrate cannot bind to the active site, or cannot be catalyzed to form a product. In a narrow range of pH, the structural and morphological changes of enzymes and substrates may be reversible.

How temperature and pH affect enzyme activity?

At low temperatures, an increase in temperature increases the rate of an enzyme-catalyzed reaction. At higher temperatures, the protein is denatured, and the rate of the reaction dramatically decreases. An enzyme has an optimum pH range in which it exhibits maximum activity.

Why does enzyme activity increase with temperature?

Higher temperature generally causes more collisions among the molecules and therefore increases the rate of a reaction. More collisions increase the likelihood that substrate will collide with the active site of the enzyme, thus increasing the rate of an enzyme-catalyzed reaction.

How pH level affect enzyme activity?

Changing the pH will affect the charges on the amino acid molecules. Amino acids that attracted each other may no longer be. Again, the shape of the enzyme, along with its active site, will change. Extremes of pH also denature enzymes.

What are 4 factors that can regulate enzyme activity?

Several factors affect the rate at which enzymatic reactions proceed – temperature, pH, enzyme concentration, substrate concentration, and the presence of any inhibitors or activators.

Why does enzyme activity decrease at high temperature?

Higher temperatures disrupt the shape of the active site, which will reduce its activity, or prevent it from working. The enzyme will have been denatured . Enzymes therefore work best at a particular temperature.