What is difference between chromatography and column chromatography?

Paper chromatography uses a cellulose paper as its stationary phase, Thin Layer chromatography uses alumina or silica gel as its stationary phase, whereas Column chromatography uses a column packed with a suitable matrix material as its stationary phase.

What do you mean by ascending paper chromatography?

The type of paper chromatography in which the solvent rises up is called Ascending paper chromatography. Alternatively, the solvent may be taken on the top in a container and be allowed to come down, in which case it is termed as Descending paper chromatography.

What is the difference between thin layer and column chromatography?

In thin layer chromatography, the stationary phase is a thin layer of silica gel or alumina on a glass, metal or plastic plate. Column chromatography works on a much larger scale by packing the same materials into a vertical glass column.

What are the different types of column chromatography?

Five chromatographic methods that use columns are gas chromatography (GC), liquid chromatography (LC), Ion exchange chromatography (IEC), size exclusion chromatography (SEC), and chiral chromatography.

What are the main differences between GC and HPLC?

The key difference between HPLC and GC is that HPLC uses a solid stationary phase and liquid mobile phase whereas GC uses a liquid stationary phase and gaseous mobile phase. HPLC and GC are both methods of separation of compounds from a mixture.

What are the two phases of paper chromatography?

Chromatography relies on two different ‘phases’:

• the mobile phase is the solvent that moves through the paper, carrying different substances with it.
• the stationary phase is contained on the paper and does not move through it.

What is difference between silica used in TLC and HPLC column?

For the VLC, generally we use a silica with a granulometry of 40-63 microns, while in the HPLC column has a granulometry of 3, 5 or 10 microns which makes we have a better separation.

What is the difference between partition and adsorption chromatography?

Adsorption chromatography is defined as a type of chromatography in which separation occurs based on adsorption. Partition chromatography is a type of chromatography in which separation is based on partition. Adsorption chromatography is a liquid-solid extraction. Partition chromatography is a liquid-liquid extraction.

What is the difference between the stationary and mobile phases in chromatography?

In all chromatography there is a mobile phase and a stationary phase. The stationary phase is the phase that doesn’t move and the mobile phase is the phase that does move. The mobile phase moves through the stationary phase picking up the compounds to be tested.

What is the difference between mobile phase and stationary phase in paper chromatography?

In paper chromatography, substances are distributed between a stationary phase and a mobile phase. The stationary phase is the water trapped between the cellulose fibers of the paper. The mobile phase is a developing solution that travels up the stationary phase, carrying the samples with it.

What is the difference between reverse phase and normal phase chromatography?

The main difference between normal phase and reverse phase chromatography is that normal phase chromatography has a very polar stationary phase and a non-polar mobile phase whereas reverse phase chromatography has a non-polar stationary phase and a polar mobile phase.

Why GC columns are longer than HPLC?

A gas encounters less resistance in the GC column which permits use of longer column lengths. Sample stability – samples analysed by HPLC are generally thermally labile so they are in liquid phase in the HPLC column at room temperatures.