What is hypoxanthine used for?

Hypoxanthine is a necessary additive in certain cell, bacteria, and parasite cultures as a substrate and nitrogen source. For example, it is commonly a required reagent in malaria parasite cultures, since Plasmodium falciparum requires a source of hypoxanthine for nucleic acid synthesis and energy metabolism.

What enzyme converts hypoxanthine?

Hypoxanthine can be converted to inosine monophosphate (IMP) by hypoxanthine-guanine phosphoribosyl transferase (HGPRT), one of the enzymes of the purine salvage pathway.

What does hypoxanthine pair with?

Hypoxanthine, a naturally occurring purine derivative, has been shown to form Watson-Crick hydrogen bonds with adenosine, cytosine, thymine and uracil, but only Hoogsteen base pairing with guanine (16, 17).

What does hypoxanthine guanine phosphoribosyltransferase do?

Purine Salvage The enzyme hypoxanthine-guanine phosphoribosyltransferase (HGPRT) is one of the central enzymes that recycle the building blocks of RNA and DNA. It attaches a purine base (either guanine or hypoxanthine, a modified form of adenine) to a sugar, creating a nucleotide.

What foods are high in hypoxanthine?

The plant source foods with the highest hypoxanthine contents were cauliflower, followed by soybeans and jengkol. The highest adenine contents were found in string beans, cauliflower and melinjo leaves.

What does hypoxanthine base pair with?

guanine
Hypoxanthine, a naturally occurring purine derivative, has been shown to form Watson-Crick hydrogen bonds with adenosine, cytosine, thymine and uracil, but only Hoogsteen base pairing with guanine (16, 17).

What is purine inhibitor?

Purine synthesis inhibitors inhibit the proliferation of cells, especially leukocytes. These inhibitors include azathioprine, an immunosuppressant used in organ transplantation, autoimmune disease such as rheumatoid arthritis or inflammatory bowel disease such as Crohn’s disease and ulcerative colitis.

How is hypoxanthine repaired?

In Escherichia coli, the major enzyme activity that initiates the repair of hypoxanthine and xanthine is endonuclease V. Endonuclease V is an endonuclease that hydrolyzes the second phosphodiester bond 3′ to the lesion.

How do you pronounce hypoxanthine?

Phonetic spelling of hypoxanthine

1. hy-pox-an-thine.
2. hahy-puh-zan-theen. Davonte Zboncak.
3. hy-pox-an-thine. Wyman Lesch.
4. hypox-anth-ine. Louise Basson.

What is hypoxanthine-guanine phosphoribosyltransferase deficiency?

Hypoxanthine-guanine phosphoribosyltransferase (HPRT) deficiency is a hereditary disorder of purine metabolism associated with uric acid overproduction and a continuum spectrum of neurological manifestations depending on the degree of the enzyme deficiency.

How does HGPRT deficiency cause gout?

The HGPRT deficiency causes a build-up of uric acid in all body fluids. The combination of increased synthesis and decreased utilization of purines leads to high levels of uric acid production. This results in both high levels of uric acid in the blood and urine, associated with severe gout and kidney problems.

Are onions high in purines?

If you have gout, dishes like chopped liver and liver and onions are best avoiding, along with other organ meats like kidney, heart, sweetbread, and tripe, since they’re high in purines.

Does allopurinol lower testosterone?

Since plasma LH (luteinizing hormone) was diminished it is concluded that allopurinol decreases the plasma testosterone level via an inhibition of LH secretion.

What is the wobble effect?

The Wobble Hypothesis explains why multiple codons can code for a single amino acid. One tRNA molecule (with one amino acid attached) can recognise and bind to more than one codon, due to the less-precise base pairs that can arise between the 3rd base of the codon and the base at the 1st position on the anticodon.