What is the conditions needed for determination of iron using 1/10-phenanthroline?

Summary. A rapid, accurate method for the determination of iron using hydroxylamine hydrochloride, sodium acetate buffer, and 1,10-phenanthroline is described. The ash is taken up with hydrochloric acid (1 + 1) and heated for 5 minutes. No interference from pyrophosphates, copper and nickel was observed.

What wavelength would you use to measure the iron phenanthroline complex?

508 nm
The determination of the iron-phen complex is performed with a spectrophotometer at a fixed wavelength of 508 nm using external calibration based on iron standard solutions.

What is the most suitable reagent for the spectrophotometric determination of iron?

Thiocyanate is one of the most important spectrophotometric reagents. The availability of the reagent and the simplicity of thiocyanate methods are responsible for its great popularity in analytical laboratories [131,132]. Thiocyanate is principally used for determination of Fe(III), Mo, W, Nb, Re, Co, U, and Ti.

What is iron phenanthroline used for?

1,10-Phenanthroline forms a stable complex with Fe(II) ion called ferroin, which is used as an indicator in Fe(II) salt titrations. Ferroin is also used in the determination of other metals, such as nickel, ruthenium, and silver.

What is the purpose for using 1/10-phenanthroline?

How do you calculate iron concentration from absorbance?

The equation should be in y=mx + b form. So if you substract your y-intercept from the absorbance and divide by the slope, you are finding the concentration of your sample.

What is spectrophotometric reagent?

Definition. A reagent used in the determination by spectrophotometry of the concentration of a chemical element or chemical compound in solution. Stars.

What is the purpose of adding 1/10-phenanthroline?

How is a spectrophotometer used in determining the water quality?

A spectrophotometer works by sending a beam of light of a specific wavelength into a cuvette containing the sample of interest suspended in a water-based solvent. Transmission spectrophotometers project light through the sample, and detectors measure the wavelength and quantity of light that pass through.

Why is pH of solution important for the determination of iron?

The most dominant form of dissolved iron is the soluble Fe+2 under the pH range of 5 to 8. Iron bacteria growth is very dependent upon the pH level, occurring over a range of 5.5 to 8.2 with 6.5 being the optimum level so it expected in all samples.

What is iron phenanthroline for?