What is the overall purpose of annexin V and propidium iodide labeling?

Annexin V and propidium iodide (PI) labeling of cells is a technique used to identify cell death, and distinguish between its different pathways: apoptosis, or programmed cell death, and necrosis.

How does annexin V PI work?

The Annexin V corresponding signal provides a very sensitive method for detecting cellular apoptosis, while propidium iodide (PI) is used to detect necrotic or late apoptotic cells, characterized by the loss of the integrity of the plasma and nuclear membranes.

What is annexin V positive?

Dual staining with fluorescent Annexin V and propidium iodide (PI) has been used to discriminate apoptotic and necrotic cell death, in which Annexin V-positive/PI-negative staining is regarded as apoptosis and PI-positive staining as necrosis.

What is Annexin V PI staining?

What is annexin V pi?

The Annexin V/ PI protocol is a commonly used approach for studying apoptotic cells3. PI is used more often than other nuclear stains because it is economical, stable and a good indicator of cell viability, based on its capacity to exclude dye in living cells 4,5.

What is the protocol for annexin V staining?

Protocol for Annexin V Staining. From the Laboratory for Cellular and Molecular Immunology, headed by Dror Mevorach, MD, at the Hadassah-Hebrew University Medical Center, Israel. This protocol is proposed for the use of annexin V staining without biasing the samples.

How do I prepare annexin V and annexin Pi?

Prepare a 10 μL solution with annexin V for a single stain, and another 10 μL solution with PI for a single stain. These will be used as single-stain controls for compensation determination, if needed.

Which annexin products can be used in parallel with annexin V-PE?

Recommended for use in parallel with Annexin V-FITC (cat. no. 556420, 556419) or Annexin V-Biotin (cat. no. 556418, 556417). Via-Probe™ (cat. no. 555816): A convenient ready-to-use solution of the nucleic acid dye 7-AAD. Recommended for use in parallel with Annexin V-PE (cat. no. 556422, 556421) or Annexin V-Biotin (cat. no. 556418, 556417).

Can annexin V be used as a probe for apoptosis?

Annexin V has a strong, Ca 2 +-dependent affinity for PS and therefore can be used as a probe for detecting apoptosis. For robust results, we recommend our range of one-step Annexin V staining / detection kits and reagents for use in flow cytometry and cell imaging.