What is the sequence of restriction enzyme?

Most restriction enzymes recognize sequences of four to eight base pairs and hydrolyze a single phosphodiester bond on each strand. A characteristic of many of these cleavage or restriction sites is their double rotational symmetry. Generally, the cleavage sites are symmetrically positioned, or palindromic.

What is SacII?

The SacII, NEB is: Time-Saver™ qualified for digestion in 5-15 minutes. 100% activity in CutSmart® Buffer (over 210 enzymes are available in the same buffer) simplifying double digests. Supplied with 1 vial of Gel Loading Dye, Purple (6X) Requires two or more sites for cleavage.

What sequence does EcoRV recognize?

Abstract. The EcoRV restriction endonuclease recognises palindromic GATATC sequences and cuts between the central T and dA bases in a reaction that has an absolute requirement for a divalent metal ion, physiologically Mg(2+).

Which restriction enzyme recognizes the DNA sequence 5 Gaattc 3 and Cleaves?

restriction endonuclease enzyme EcoRI
The restriction endonuclease enzyme EcoRI recognizes the ssDNA sequence 5′-GAATTC’-3, and introduces a single-strand cut between the G & A nucleotides.

What sequence is cut by Hind 2?

Hind II was the first discovered restriction endonuclease enzyme. It has been isolated from Haemophilus influenzae Rd. It cuts DNA molecules at a particular point by recognising a specific sequence of six base pairs. This specific sequence is known as the recognition sequence for Hin d II.

What is the recognition sequence for BamHI?

BamHI binds at the recognition sequence 5′-GGATCC-3′ , and cleaves these sequences just after the 5′-guanine on each strand. This cleavage results in “sticky ends” which are 4 b.p. long.

Is Hind 3 a restriction endonuclease?

HindIII (pronounced “Hin D Three”) is a type II site-specific deoxyribonuclease restriction enzyme isolated from Haemophilus influenzae that cleaves the DNA palindromic sequence AAGCTT in the presence of the cofactor Mg2+ via hydrolysis.

What is the palindromic sequence for Hind 2?

Hind II recognizes the sequence GTPy/PuAC and generates fragments with blunt ends (1).

What does EcoRV cut?

Uses. EcoRV is often used to cut open a plasmid vector to insert a gene-of-interest during gene cloning. The enzyme is supplied by many manufacturers and requires bovine serum albumin to work properly.

What kind of nucleases are BamHI and EcoRI?

BanmHI is a type II restriction endonuclease (from Bacillus amyloliquefaciens H) that binds as a dimer to the symmetrical sequence 5′-GGATCC-3′[10]. The BamHI recognition sequence differs by only one base pair in each half site from the EcoRI sequence 5′-GAATTC-3′.